This article describes the capillary zone electrophoresis analysis of neuropeptides. The method allows for rapid, sensitive, and simultaneous identification and quantification of diastereomeric peptides.
The document details an analytical method for separating neurotensin, angiotensin I, angiotensin II, and [Leu]enkephalin. It highlights the ability of capillary zone electrophoresis (CZE) to rapidly identify and quantify peptide diastereomers based on their differing physicochemical properties.
The analysis separated neurotensin, angiotensin I, angiotensin II, and [Leu]enkephalin.
The analysis used a 30 mM borate buffer at pH 8.3.
The capillary temperature was maintained at 30°C.