This note introduces the methods for chiral separations by CE, including theory, method development strategies, experimental procedures etc.
This document outlines the theory and practical methodology for chiral separations using capillary electrophoresis (CE) with cyclodextrins. It explains the mechanism of chiral recognition, where analytes form transient diastereomeric complexes with optically pure additives like cyclodextrins dissolved in the buffer electrolyte. The note details how parameters such as cyclodextrin type, concentration, buffer pH, and temperature influence the separation of enantiomers.
This note is meant to facilitate the development of chiral separations by capillary electrophoresis.
All cyclodextrins should be used at a nominal concentration of 20 mM during the screening step.