A method for determining the residues of melamine in milk powder and egg
This document details a method for determining melamine residues in milk powder and egg, developed to comply with Chinese regulatory standards (GB/T 22388-2008). The procedure utilizes Agilent SampliQ Polymer SCX Solid Phase Extraction for sample preparation, followed by separation and detection using the Agilent 1200 Series HPLC with a diode array detector.
The method achieves a limit of detection of 10 pg/kg and demonstrates linear calibration curves over the range of 1 to 20 mg/kg. Recoveries range from 84 to 96 percent with relative standard deviations below 3.0 percent, making it suitable for food safety analysis.
| Limit of Detection | 10 pg/kg |
|---|---|
| Linear Calibration Range | 1 to 20 mg/kg |
| Overall Recoveries | 84 to 96 percent |
| RSD Values | below 3.0 percent |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 40 °C |
| Detector Wavelength | 240 nm |
The limit of detection is 10 pg/kg.
Linear calibration curves were obtained over the range of 1 to 20 mg/kg.
Overall recoveries range from 84 to 96 percent.