A high performance liquid chromatography tandem mass spectrometric (HPLC/MS/MS) method was developed for the simultaneous determination of 10 antibiotic residues: minocycline, 4-epioxytetracycline, 4-epitetracycline, tetracycline, 4-epichlortetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline, oxytetracycline in milk and animal tissues. In the method, Agilent’s novel solid phase extraction cartridge and a reversed phase Agilent ZORBAX RX C8 column (5 µm, 150 mm × 2.1 mm) were used for purification and separation. The limit of detection (LOD) is between 0.5 and 10.0 µg/kg and the limit of quantitation (LOQ) is less than 50 µg/kg. The linearity is obtained from 5 to 1000 µg/kg. Overall recoveries are between 76.4% and 101% with a relative standard deviation (RSD, n = 6) less than 8.4%. The method is rapid, sensitive, convenient and robust, and can be used to simultaneously confirm multi-residues of tetracyclines and their metabolites in milk.
This document details a High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC/MS/MS) method developed to simultaneously determine ten antibiotic residues, specifically tetracyclines and their metabolites, in milk and animal tissues. The study utilizes the Agilent 6410 LC/MS/MS system along with a ZORBAX RX C8 column and SampliQ OPT solid phase extraction cartridges.
The limit of detection (LOD) is between 0.5 and 10.0 yg/ kg.
The total run time is 28 minutes.
A reversed phase Agilent ZORBAX RX C8 column (5 um, 150 mm x 2.1 mm) is used.