The method is used for the simultaneous determination of three antibiotic residues of chloram- phenicol (CAP), florfenicol (FF), and thiamphenicol (TAP) in honey.
This document details a method for the simultaneous determination of three antibiotic residues—chloramphenicol, florfenicol, and thiamphenicol—in honey. The procedure utilizes liquid-liquid extraction followed by solid-phase extraction (SPE) using Agilent SampliQ OPT cartridges to purify the analytes. Quantification is performed using liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in negative ion multiple reaction monitoring mode.
The method was validated to achieve reproducible quantitative results with limits of quantitation in the sub-ng/g to ng/g range. Overall recoveries ranged from 74.9% to 107%, and the dynamic calibration ranges were established for all three antibiotics. The document provides specific experimental conditions, including reagent preparation, sample preparation steps, instrument settings for the Agilent 1200 Series HPLC and 6410 Triple Quadrupole LC-MS/MS system, and chromatographic parameters.
| Limit of Quantitation (LOQ) for Thiamphenicol | 1.0 ng/g |
|---|---|
| Limit of Quantitation (LOQ) for Chloramphenicol | 0.1 ng/g |
| Limit of Quantitation (LOQ) for Florfenicol | 0.1 ng/g |
| Column Dimensions | 150 mm x 2.4 mm, 5 um |
| Flow Rate | 0.3 mL/min |
| Column Temperature | 30°C |
| Injection Volume | 20 uL |
The method determines chloramphenicol, florfenicol, and thiamphenicol residues in honey.
The limit of quantitation found for thiamphenicol is 1.0 ng/g.
Agilent SampliQ OPT solid-phase extraction cartridges are used for purification.