This application note describes the utility of phenyl columns using 100% aqueous mobile phases without the inconvenience of phase collapse. The selectivity of different Agilent ZORBAX phenyl phases (StableBond SB-Phenyl, Eclipse XDB-Phenyl and Eclipse Plus Phenyl-Hexyl) is demonstrated with both aliphatic acids and catecholamines (bases).
The document presents a study by Agilent Technologies demonstrating that phenyl columns can effectively separate polar compounds like acids and bases without requiring organic solvents or ion-pairing reagents. This approach avoids issues such as phase collapse often seen with alkyl columns in low-organic mobile phases.
The note details experimental results using the Agilent 1200 Series Rapid Resolution LC system to analyze catecholamines (bases) and aliphatic acids. It compares the selectivity of three specific column types: ZORBAX Eclipse Plus Phenyl-Hexyl, ZORBAX Eclipse XDB-Phenyl, and ZORBAX StableBond SB-Phenyl.
This application note demonstrates how to analyze polar compounds like acids and bases using phenyl columns with a 100% aqueous mobile phase.
The standard column used has a diameter of 4.6 mm, a length of 100 mm, and a particle size of 5 microns.
The analysis was performed using a mobile phase flow rate of 1 mL/min.