Agilent Technologies' new fast GC/MSD method can significantly speed up pesticide screening
Agilent Technologies developed a new, fast GC/MSD method to significantly speed up the screening of pesticides and endocrine disrupters. The method utilizes Agilent's Method Translation (MTL) and Retention Time Locking (RTL) software to scale a standard 42-minute analysis down to as little as 10.5 minutes without losing resolution. A specific combination of column stationary phase, carrier gas flow rate, and oven temperature programming is required to lock all compounds to an expected retention timetable.
The document details the experimental setup, including the use of a 10 m x 0.1 mm x 0.1 µm HP-5 column and a 16:1 split ratio. It presents chromatographic conditions for onefold, twofold, threefold, and fourfold speed gains in a table. The results show that the scaled screening libraries can accurately correspond to the faster analyses, making the screening process more powerful and adaptable.
| Analysis time (standard) | 42 minutes |
|---|---|
| Minimum analysis time | 10.5 minutes |
| Column length | 10 m |
| Column inner diameter | 0.1 mm |
| Column film thickness | 0.1 µm |
| Split ratio | 16:1 |
| Carrier gas | Helium |
| Injector temperature | 250°C |
| Source temperature | 230°C |
| Transfer line temperature | 280°C |
| High mass | 500 amu |
Agilent's GC method translation software (MTL) and retention time locking (RTL) software were used.
The method screens for 567 of the most common pesticides and endocrine disrupters.
A 10 m x 0.1 mm x 0.1 µm HP-5 column was used.