This document describes a method for extracting 壬基酚 from water samples using the AccuBOND II ODS (C18) 6 mL/500 mg cartridge.
This Application Brief details the Solid Phase Extraction (SPE) procedure for chlorinated pesticides in water using the Agilent AccuBOND ODS (C18) cartridge. The process involves conditioning the cartridge with acetone, methanol, and water, followed by loading the sample, rinsing, and eluting the analytes with acetone for concentration and GC injection.
The document includes specific instructions for equipment setup, reagent preparation, and step-by-step operational procedures. It also presents chromatographic data for a standard mixture of chlorinated pesticides, listing retention times, recovery percentages, and standard deviation for various compounds using a DB-608 column.
| Cartridge Type | AccuBOND ODS (C18) 6 mL/ 500 mg |
|---|---|
| Column Type | DB-608 |
| Column Dimensions | 30 m x 0.53 mm I.D. |
| Film Thickness | 0.83 pm |
| Carrier Gas | Helium |
| Flow Rate | 40 cm/sec |
| Injector Temperature | 250°C |
| Detector Temperature | 325°C |
The flow rate should be no greater than 10 mL/min.
Concentrate to dryness under a stream of dry nitrogen.
Using heat to aid in sample concentration may result in reduced recovery values.