Agilent 2100 Bioanalyzer Application Note: Detecting Genetically Modified Organisms

About this manual

This document details a method developed by Genolife to detect genetically modified organisms (GMOs) in food, specifically focusing on Roundup Ready soy (RRS) and various corn transgenes. The study utilizes the Agilent 2100 bioanalyzer with the DNA 500 LabChip kit to analyze multiplex PCR products for transparency and labeling compliance.

What's inside

• Abstract
• Introduction
• GMO maize detection in food ingredients by multiplex PCR
• Results and discussion
• RRS detection in food ingredients
• Conclusion

Frequently asked questions

What is the detection limit for Roundup Ready soy in food ingredients?

The method can detect as little as 0.01% of Roundup Ready soy in food ingredients.

What are the copy number limits for detecting specific corn transgenes?

The multiplex PCR detects 10 copies of MON810, GA21 and Bt11, and 100 copies of Bt176 and T25.

What size range of DNA fragments can the Agilent 2100 bioanalyzer analyze with the DNA 500 LabChip kit?

The kit allows analysis of DNA fragments ranging in size from 25 to 500 bp.