This document describes the use of liquid chromatography/mass selective detection (LC/MSD) technique to replace the nonspecific post-column derivatization process for detecting low ppb levels of carbamate pesticides in food. System linearity, sensitivity, and repeatability were evaluated on nine carbamates, and the instrument detection limits were determined to be about 3 to 10 ppb for all the analytes in broccoli extract. This method overcomes the false positive issue of fluorescence detection method, providing higher specificity, no additional hardware and plumbing requirements, and lower limits of detection.
This document details a method for detecting carbamate pesticides in food samples, such as broccoli extract, using liquid chromatography with mass selective detection (LC/MSD). The study evaluates the system's linearity, sensitivity, and repeatability for nine specific carbamate analytes without the need for post-column derivatization. Results indicate that the LC/MSD method provides detection limits between 3 and 10 ppb, offering a viable alternative to fluorescence detection methods.
| Instrument System | Agilent 1100 MSD SL Quad system |
|---|---|
| Flow Rate | 0.2 mL/min |
| Ionization Mode | Positive electrospray |
| Scan Range | 100 to 500 |
| Drying Gas Flow | 10 L/min at 350 °C |
| Detection Limits | 3 to 10 ppb |
The instrument detection limits were determined to be about 3 to 10 ppb for all the analytes in broccoli extract.
Setting the fragmentor to either 60 or 80 V will have the best results.
The correlation coefficients of all analytes are greater than 0.998 for the range from 0.2 to 1000 ppb.