Sugars and sugar alcohols were successfully analyzed by negative ion mode of liquid chromatography/mass spectrometry using atmospheric pressure chemical ionization
This application note details a method for the analysis of sugars and sugar alcohols using Liquid Chromatography/Mass Spectrometry (LC/MS). The method utilizes atmospheric pressure chemical ionization (APCI) in negative ion mode, which allows for the analysis of these compounds without the need for preliminary derivatization, unlike other methods such as GC/MS or standard HPLC.
| Instrument | HP 1100 LC/MS with APCI |
|---|---|
| Ion Mode | Negative ion mode |
| Drying gas flow | 1.3 L/min |
| Nebulizer pressure | 40 psi |
| Fragmentor voltage | 20V |
| Corona current | 30 pA |
| Mass range | 100-500 m/z |
| Flow rate | 0.2 mL/min |
| Oven temperature | 40 C |
| Injection volume | 10 uL |
The APCI method employing post-column addition has a lower sensitivity of several hundred picograms, though derivatization methods can reach several to tens of picograms.
The method successfully analyzes sorbitol, glucose, fructose, xylitol, and sucrose.
The instrument uses a drying gas of 13 L/min at 350 °C, a nebulizer of 40 psi, and a fragmentor of 20V.
The mobile phase is CH3CN/H2O (75/25) at 0.2 mL/min, and the column is Asahipak NH2-50 2D, 2.0 mm id x 150 mm long.