This Application Note investigates the ability of a novel polymeric mixed mode strong anion exchange resin to extract acidic, neutral and basic drugs from urine
The document describes a study evaluating the performance of the Agilent SampliQ-SAX mixed-mode strong anion exchange polymeric resin. The goal was to extract acidic, basic, and neutral drugs from a complex biological matrix (urine) simultaneously.
Four specific drugs were used as representatives for each class: secobarbital (neutral), nortriptyline (basic), ketoprofen (acidic), and naproxen (acidic). The study details the sample preparation protocol, including hydrolysis, pH adjustment, loading onto SPE cartridges, and sequential elution steps to separate the drug classes. High recoveries and excellent reproducibility were achieved for all analytes.
| SPE Cartridge Volume/Capacity | 1-mL/30 mg |
|---|---|
| Flow Rate | 1.5 mL/min |
The study used secobarbital (neutral), nortriptyline (basic), ketoprofen (acidic), and naproxen (acidic) as representatives for the three classes of drugs.
The limits of detection were 0.21 pg/mL for secobarbital, 0.04 pg/mL for nortriptyline, 0.03 pg/mL for ketoprofen, and 0.03 pg/mL for naproxen.