This Application Note describes a method for the long-term detection of anabolic steroid metabolites in urine using both the Agilent 6890N network GC system with an Agilent 5975B Series GC/MSD and the Agilent 7000 Series Triple Quadrupole GC/MS. Monitoring the sulfate conjugates of these metabolites, instead of the routine glucuronide metabolites, allowed detection of methenolone for a post-administration period that was almost twice as long. This was achieved using GC/MS in SIM mode. Performing the analysis on the Triple Quadrupole GC/MS in SRM mode extends the detection period even further, to almost three times as long as the conventional glucuronide approach.
This document details a method for the long-term detection of anabolic steroid metabolites in urine, developed using Agilent 6890N network GC with 5975B GC/MSD and Agilent 7000 Series Triple Quadrupole GC/MS. The approach focuses on monitoring sulfate conjugates of metabolites rather than the routine glucuronide metabolites, which allows for a significantly extended detection window. Specifically, this method enables the detection of methenolone for a post-administration period almost twice as long using the 6890N system, and nearly three times as long using the 7000 Series system.
| Analytical column | Custom HP-1ms 10 m x 0.15 mm, 0.12 um film |
|---|---|
| Carrier gas | Hydrogen, Constant Flow, 1.0 mL/min |
| Source temperature | 230 °C |
| Quadrupole temperature | 150 °C |
| Transfer line temp | 280 °C |
The sulfate-conjugated metabolite can be detected for at least nine days, whereas the commonly targeted methenolone metabolites in the glucuronide fraction were detectable for only five days.
The analysis was performed on an Agilent 6890N network GC system with an Agilent 5975B Series GC/MSD and the Agilent 7000 Series Triple Quadrupole GC/MS.