Agilent Poroshell 120 columns offer the highest efficiency, while significantly reducing back pressure
This document is an application note by Agilent Technologies demonstrating the efficiency of Poroshell 120 superficially porous columns. It shows that coupling three 150 mm columns can achieve over 100,000 plates in less than 5 minutes while keeping back pressure manageable. The note compares these columns to sub-2-µm particle columns, highlighting lower back pressure and higher peak capacity.
| Particle size | 2.7 µm |
|---|---|
| Core size | 1.7 µm |
| Shell size | 0.5 µm |
| Back pressure reduction | 40-50% lower |
| Efficiency comparison | 80-90% of sub-2-µm |
These columns can be used up to 600 bar.
Approximately 103,000 plates were obtained at 1.5 mL/min flow rate.
The Poroshell 120 column showed 133 peaks, which is 30% higher than the sub-2-µm column's 101 peaks.