This application note describes a new EPA drinking water LC/MS/MS method for the determination of non-volatile compounds on the Varian 325-MS triple quadrupole mass spectrometer.
This application note evaluates a new U.S. EPA method for determining semi-volatile and non-volatile contaminants in drinking water using the Varian (now Agilent) 325-MS triple quadrupole mass spectrometer equipped with a vortex ESI (vESI™) source.
The study details the optimization of LC/MS/MS conditions, including HPLC parameters and MS settings such as gas temperatures and pressures. It demonstrates how programming these parameters using SelecFlow™ and SelecTemp™ features improves sensitivity for various analytes compared to fixed temperature settings.
The vortex ESI (vESI™) source is used together with the ability to program temperature and flow rate of atmospheric pressure interface gases.
The lowest calibration point must have +50% recovery, and all other levels must have +30% recovery.