This document describes a simple, high-throughput LC/MS/MS method for the detection and quantitation of (±)-11-nor-9-carboxy-delta-9-THC in urine using negative ion electrospray. The method does not require derivatization and provides the same specificity and sensitivity as GC/MS.
The document details an analytical method using the Varian (now Agilent) 1200L LC/MS/MS system equipped with an ESI source to analyze urine samples for the THC metabolite (+)-11-nor-9-carboxy-delta-9-THC. The method utilizes negative ion electrospray ionization and eliminates the need for time-consuming sample derivatization required by traditional GC/MS techniques.
Specific instrumentation and conditions are provided, including the Varian ProStar 410 AutoSampler, ProStar 210 Solvent Delivery Modules, and a Varian Pursuit Diphenyl column. The method achieves a linear range from 1 ppb to 1000 ppb with a retention time of 4.6 minutes for the analyte.
| Column | Varian Pursuit Diphenyl 3 um, 50 x 2 mm |
|---|---|
| Linear Range | 1 ppb to 1000 ppb |
| Retention Time | 4.6 minutes |
The method is linear from 1 ppb to 1000 ppb.
No, the LC/MS/MS method provides specificity and sensitivity without the need for a derivatization process.
The retention time for the THCC analyte is 4.6 minutes.