This method is applicable to a wide range of animal and plant tissues including dairy products, grains, fruit and vegetables and oil seeds such as poppy seeds. It is suitable for cadmium concentration in the range 0.01-0.3 µg/g. Sample is digested by wet ashing and cadmium is then complexed and extracted into an organic solvent. After removal of the spent aqueous phase the organo metallic complex is destroyed by the addition of dilute HNO3. This aqueous phase containing the stripped cadmium is then analyzed by Graphite Furnace Atomic Absorption Spectroscopy (GFAAS). The additional stripping step is employed to improve precision and facilitate the use of aqueous standards.
This document outlines a procedure for determining cadmium concentrations in animal and plant tissues, including dairy products, grains, fruits, vegetables, and oil seeds. The method is suitable for cadmium concentrations in the range of 0.01-0.3 µg/g. The process involves digesting samples via wet ashing, complexing cadmium with ammonium pyrrolidine dithiocarbamate (APDC), and extracting it into di-isobutyl ketone (DIBK). The organic phase is then stripped with dilute nitric acid, and the resulting aqueous phase is analyzed using Graphite Furnace Atomic Absorption Spectroscopy (GFAAS).
| Applicable Cadmium Concentration Range | 0.01-0.3 µg/g |
|---|---|
| Instrumentation Used | Agilent GTA 95 with Agilent AA-875 |
| Detection Limit (Solution) | Better than 0.0005 mg/mL Cd |
| Reporting Limit (Grains) | 0.02 mg Cd/g |
The method is suitable for cadmium concentration in the range 0.01-0.3 µg/g.
An Agilent GTA 95 graphite furnace atomizer with autosampler is used with an Agilent AA-875.
The detection limit is better than 0.0005 mg/mL Cd in solution.
The recovery should be between 80% and 120%.