This application note describes the method for analysis of fragrance and allergens using Agilent J&W FactorFour VF-WAXms GC column.
This document details the analysis of a standard fragrance mix containing eight common allergens, including oak moss extract, iso-eugenol, eugenol, cinnamal, hydroxy citronellal, geraniol, cinnamyl alcohol, and amyl cinnamal. The text highlights the suitability of the VF-WAXms column for this application, noting its MS compatibility and low bleed profile around 250 °C.
| Column Dimensions | 30 m x 0.25 mm x 0.25 µm |
|---|---|
| Oven Temperature Range | 100 °C to 250 °C |
| Oven Ramp Rate | 10 °C / min |
| Carrier Gas | Helium |
| Carrier Gas Flow Rate | 1.0 mL/min |
| Injector Temperature | 250 °C |
| Detector Type | GC-MS Iontrap |
| Trap Temperature | 200 °C |
| Manifold Temperature | 60°C |
| Sample Size | 0.1 µL |
| Sample Concentration | 500 ppm |
The mix includes oak moss extract, iso-eugenol, eugenol, cinnamal, hydroxy citronellal, geraniol, cinnamyl alcohol, and amyl cinnamal.
The oven should be set from 100 °C to 250 °C with a ramp rate of 10 °C / min.