This application note describes an LC/MS compatible method for the separation of cold/cough medication using the Polaris™ 5µ C18-A column. The ingredients included are acetaminophen, pseudoephedrine, chlorpheniramine maleate, dextromethorphan and unknown components.
The document presents Agilent Technologies Application Note 1931, which details an LC/MS compatible High-Performance Liquid Chromatography (HPLC) method. The method is designed for the separation of active ingredients found in children's cold and flu medications.
The procedure utilizes a Polaris™ 5µm G18-A column with dimensions of 50 x 4.6 mm. The mobile phase consists of two eluents: A (H2O + 0.1% TFA) and B (MeOH + 0.1% TFA), with a gradient increasing from 10% to 95% B over 5 minutes at a flow rate of 2.0 ml/min. Detection is performed using UV at 214 nm.
| Column | Polaris™ 5µm G18-A, 50 x 4.6 |
|---|---|
| Eluent A | H2O + 0.1% TFA |
| Eluent B | MeOH + 0.1% TFA |
| Gradient | 10 to 95%B in 5 min |
| Flow rate | 2.0 ml/min |
| Detection wavelength | UV @ 214nm |
It provides an LC/MS compatible method for separating cold and cough medicine components.
The method uses a Polaris™ 5µm G18-A, 50 x 4.6 column.
Eluent A is H2O + 0.1% TFA and Eluent B is MeOH + 0.1% TFA.