This article presents a method for the USP assay of ibuprofen oral suspension using Varian 920-LC and Pursuit™ XRs C8 column. The results obtained from the experiment show that all performance requirements of the USP methods are satisfied and demonstrate a significant enhancement in laboratory productivity when using this system.
This document describes a productivity-enhanced High-Performance Liquid Chromatography (HPLC) method for the assay of ibuprofen in oral suspension, complying with United States Pharmacopoeia (USP) standards. The method utilizes the Varian 920-LC liquid chromatograph equipped with a diode array detector and an autosampler. The study compares a standard method using a 150x4.6 mm column with a faster method using a shorter 50x4.6 mm column to improve sample throughput.
| Column (Fast Method) | Pursuit XRs C8, 3 um, 50x4.6 mm |
|---|---|
| Column (Standard Method) | Pursuit XRs C8, 5 um, 150x4.6 mm |
| Mobile Phase | 0.01 M Phosphoric Acid: Acetonitrile (55:45 % v/v) |
| Flow Rate | 2 mL/min |
| Wavelength | 220 nm |
The run time can be reduced to below 5 minutes using the shorter column.
Yes, the performance surpasses the USP method requirements for resolution, tailing, and precision.
The experiment used a Varian 920-LC liquid chromatograph with a low pressure quaternary gradient pump.