Agilent Application Note 1927 describes a method for separating codeine and ibuprofen on a Polaris C8-A column.
This document is an application note from Agilent Technologies detailing the high-performance liquid chromatography (HPLC) separation of the drugs codeine and ibuprofen. The method utilizes a Polaris C8-A column with specific eluent conditions.
The separation was performed using a mobile phase consisting of 10 mM ammonium acetate at pH 8.0 and acetonitrile (MeCN) in a 62:38 ratio. The flow rate was set to 1.6 ml/min at ambient temperature, with detection occurring at 210 nm.
| Column | Polaris C8-A 5y, 50 x 4.6mm |
|---|---|
| Part Number | A2010050X046 |
| Eluent | 10 mM Ammonium acetate, pH 8.0: MeCN, 62:38 |
| Flow Rate | 1.6 ml/min |
| Temperature | ambient |
| Detection | 210 nm |
The flow rate is 1.6 ml/min.
The temperature is ambient.
Detection is performed at 210 nm.