This document describes a method for quantitative analysis of the herbicide imazaquin on the Varian 320-MS Triple Quadrupole LC/MS/MS. The method is highly sensitive, selective and accurate, and can detect imazaquin at trace levels.
The document details a method developed to quantify the concentration of the imidazolinone herbicide imazaquin at trace levels. The analysis utilizes an ESI positive ionization mode and specific HPLC conditions, including a Polaris C18-A column and a mobile phase of 60:40 water:methanol with 0.1% formic acid.
The method can detect and quantify imazaquin from 0.05 ppb to 200 ppb.
The calibration curve for imazaquin has an r value of 0.999.
An RMS signal-to-noise ratio of 61 was obtained for a 20-pL injection of a 0.05 ppb standard.