This application note describes a method for the detection of oxytetracycline and chlorotetracycline in animal feed and tissue extracts using the 320-MS Triple Quadrupole Mass Spectrometer.
This document presents an LC/MS/MS methodology for analyzing oxytetracycline (OTC) and chlorotetracycline (CTC) in animal feed and tissue extracts. It details the use of the Agilent 320-MS Triple Quadrupole Mass Spectrometer equipped with an ESI source to detect these antibiotics, which are widely used in food-producing animals.
| Instrument Model | Varian 320-MS Triple Quadrupole Mass Spectrometer |
|---|---|
| Nebulizing Gas Pressure | 55 psi |
| Drying Gas Pressure | 36 psi at 400 °C |
| Column Temperature | 45°C |
| Calibration Range (Feed) | 0.2 - 20 ng/uL |
| Calibration Range (Tissue) | 0.02 - 2.0 ng/uL |
The document describes the analysis of oxytetracycline (OTC) and chlorotetracycline (CTC).
The methodology is applied to animal feed extracts and chicken breast tissue extracts.