Agilent 320-MS LC/MS/MS Application Note for Aflatoxin Analysis

About this manual

The document presents a validated Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS) method for the analysis of carcinogenic mycotoxins known as aflatoxins. The method utilizes an Agilent 320-MS instrument equipped with an ESI source and ProStar solvent delivery systems to detect aflatoxins B1, B2, G1, and G2 in complex food matrices such as sake and wheat beer.

The procedure involves sample preparation using the AflaCLEAN immunoaffinity column for cleanup. The method details specific HPLC conditions, API parameters including ionization mode and gas settings, and MRM (Multiple Reaction Monitoring) parameters for quantitation and confirmation of each toxin. Results indicate that all four aflatoxins can be separated and detected in less than eight minutes with limits of quantitation below 0.5 ppb.

What's inside

• Introduction
• Instrumentation
• Sample Preparation
• HPLC Conditions
• API Conditions
• MRM Parameters
• Results & Discussion
• Validation of the method

Frequently asked questions

What aflatoxins does this method detect?

The method detects and analyzes aflatoxins B1, B2, G1, and G2.

What is the limit of quantitation (LOQ) for this method?

The LOQs for all four aflatoxins are less than or equal to 0.5 ppb.

Which instrument is used in this application note?

The analysis was performed using a Varian (now Agilent) 320-MS LC/MS/MS system.