This document introduces rapid-fire electron capture dissociation for post-translational modification studies of proteins and peptides.
This document details the application of Electron Capture Dissociation (ECD) using the Agilent Rapid-Fire ECD Module combined with the 901-MS (QFT) 7.0 T instrument. It explains the mechanism of ECD, highlighting its ability to retain labile post-translational modifications (PTMs) like phosphorylation during fragmentation, which is advantageous for top-down proteomics.
A single electron beam pulse of 30 to 50 ms duration is typically sufficient for extensive fragmentation.
The average mass error for the identified fragments was 0.98 ppm for this experiment.
Twenty scans were accumulated for the electron beam fired for 50 ms.