This document describes a method for the analysis of morphine and other opiates in urine using a new derivatization method that converts keto-opiates to their oxime derivatives during glucuronide hydrolysis with subsequent TMS derivatization to meet all our requirements for opiate GC/MS analysis.
This document outlines a robust gas chromatography-mass spectrometry (GC/MS) method for confirming the presence of morphine and other opiates in urine samples. The method addresses the difficulty of separating common opiate drugs by utilizing a specific derivatization process involving conversion to oxime derivatives followed by TMS derivatization. This approach allows for the resolution and identification of codeine, morphine, hydromorphone, 6-monoacetyl-morphine, hydrocodone, and oxycodone in a single extraction and injection.
| Column | DB-Sms (J&W) 30m x 0.25 mm x 0.25um |
|---|---|
| Injection Temperature | 170°C |
| Transfer Line Temperature | 280°C |
| Mass Range | 280-480 |
| Scan Speed | 0.6 Sec/scan |
| Filament Current | 100 up amps |
| Background Mass | 249 |
| AGC Target | 10000 |
The method resolves and identifies codeine, morphine, hydromorphone, 6-monoacetyl-morphine, hydrocodone, and oxycodone.
The sample should be placed in a heater block for 2 hours at 55°C.
The residue is capped and heated for 30 minutes at 70°C after adding BSTFA + 10% TMCS.