Agilent Bravo for Protein Purification Application Note: High Throughput IgG Purification

About this manual

The document details a system called the Agilent Bravo for Protein Purification, which combines the 96-channel liquid handling head of the Agilent Bravo platform with AssayMAP protein purification cartridges from BioSystem Development. This setup enables microchromatography on complex samples, allowing for the purification of immunoglobulin G (IgG) in high-throughput formats up to 96 samples per run.

What's inside

• Abstract
• Introduction
• Materials and Methods
• Workflow
• Results and Discussion
• Conclusion

Frequently asked questions

What is the maximum amount of non-specific protein the system can handle in a sample background?

The system can purify target protein when presented in backgrounds as high as 10 mg/mL of non-specific protein.

What is the maximum number of samples that can be processed in a single run?

The automated platform allows for multiplexing up to 96 samples in a single run.