Agilent Immunoprecipitation combined with microchip capillary gel electrophoresis for detection quantification of B-galactosidase from crude E. coli cell lysate in bioprocess technology

Update: 30 September, 2023

This Application Note describes the development of an immunoprecipitation method in combination with microfluidic on-chip analysis on the Agilent 2100 Bioanalyzer for sensitive, selective and quantitative detection of endogenous β-galactosidase in crude E. coli lysates. The effects of antibody selection and the importance of preclearing steps for sensitive and specific detection of the target protein are discussed. Quantification of β-galactosidase was achieved via an external calibration curve based on various spiking levels of pure β-galactosidase into E. coli lysate. Results proved the described approach to be quantitative over orders of magnitudes and highly reproducible.

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Publication date: 11 June, 2012

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