Two samples consisting of various nonsteroidal anti-inflammatory drugs (NSAID) are separated using methods developed on an Agilent 1200 Series Method Development Solution controlled by ACD/Labs AutoChrom for ChemStation software. The chromatograph while under control of AutoChrom can screen up to seven columns, thirteen buffers and two organic solvents with columns held in four temperature controlled zones. The software helps plan the next best experiment to perform, allowing the analyst to focus on quickly developing methods using conditions with the best likelihood of success. In this work four columns (Agilent ZORBAX StableBond SB-C18, Agilent ZORBAX Eclipse Plus C18, Agilent ZORBAX Bonus RP and Agilent ZORBAX Eclipse Plus Phenyl-Hexyl) are screened using five mobile phase modifiers. Twenty solvent column experiments are initially screened for each sample set.
The document details a method development strategy for separating various nonsteroidal anti-inflammatory drugs (NSAIDs). It utilizes an Agilent 1200 Series LC system controlled by ACD/Labs AutoChrom software to screen multiple columns and mobile phase modifiers efficiently.
Four specific Agilent ZORBAX RRHT columns were evaluated: StableBond SB-C18, Eclipse Plus C18, Bonus RP, and Eclipse Plus Phenyl-Hexyl. The study screened these columns against five different mobile phase modifiers, including trifluoroacetic acid, formic acid, acetic acid, and ammonium acetate buffers at pH 4.8 and 6.5.
The G1312B Binary Pump SL used in the system is capable of delivering up to 600 bar.
The experiments were conducted with a temperature controlled at 25 °C and a flow rate set at 2 ml/min.
The system can hold up to eight columns, but only seven are supported when the AutoChrom software is used.