This Application Note describes the performance comparison and benchmark of the Agilent 2200 TapeStation system with the Agilent 2100 Bioanalyzer system, the industry standard for RNA quality control. The 2200 TapeStation system performs automated analysis of up to 96 samples with approximate analysis time of 1 minute per sample. This Application Note demonstrates the high correlation between RINe generated by the 2200 TapeStation system with the RIN quality assessment of the 2100 Bioanalyzer system. The high reproducibility of RINe achieved with the 2200 TapeStation system in RNA quality assessment is also shown. The 2200 TapeStation system is, therefore, a reliable system for RNA quality control.
This document serves as an application note comparing the performance of the Agilent 2200 TapeStation system with the Agilent 2100 Bioanalyzer system for RNA quality control. It highlights that the 2200 TapeStation performs automated analysis of up to 96 samples with an approximate analysis time of 1 minute per sample using ScreenTape consumables.
The study evaluates the correlation between the RNA Integrity Number (RIN) generated by the 2200 TapeStation and the RIN obtained from the 2100 Bioanalyzer. Results indicate a high correlation (R² values of 0.9878 and 0.9474) and high reproducibility, with intra-assay precision better than 2% to 4% depending on the kit used. The document concludes that the 2200 TapeStation is a reliable system for RNA quality control due to its high throughput, ease of use, and comparable performance to the industry standard.
| Maximum sample throughput | up to 96 samples |
|---|---|
| Approximate analysis time | 1 minute per sample |
| RIN range | 1 to 10 |
The approximate analysis time is 1 minute per sample.
RIN is presented as a value between 1 and 10, where 10 represents the highest quality.
The 2200 TapeStation shows higher precision due to automated sample handling and loading that reduces manual pipetting errors.