A method for simultaneous determination of malachite green and crystal violet, and their metabolites leuco-malachite green and leuco-crystal violet in fish, was developed and validated. The analytes were extracted by solid phase extraction and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry operating in positive ion multiple reaction monitoring mode. The method provided a 0.5 ng/g limit of quantification for all these compounds in fish. The dynamic calibration ranges for these compounds were obtained from 0.5 to 100 ng/g. The overall recoveries ranged from 96 to 109% with RSD values between 1.7 and 4.5%.
The document presents a developed and validated analytical method for the simultaneous determination of malachite green (MG), crystal violet (CV), and their metabolites leuco-malachite green (LMG) and leuco-crystal violet (LCV) in fish tissue. The analytes were extracted using solid phase extraction with Agilent Bond Elut Plexa PCX cartridges and quantified via liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC/MS/MS).
The method provides a 0.5 ng/g limit of quantification for all tested compounds.
The dynamic calibration ranges were obtained from 0.5 to 100 ng/g.
Overall recoveries ranged from 96 to 109% with RSD values between 1.7 and 4.5%.