This article describes an LC/MS/MS method for the determination of buprenorphine and norbuprenorphine in whole blood using an Agilent Bond Elut Plexa PCX and an Agilent Poroshell 120 column
This document details an analytical method for detecting buprenorphine and norbuprenorphine in whole blood samples using Agilent LC/MS/MS technology. The method achieves a limit of detection below 0.1 ng/mL by combining a specific sample cleanup procedure with high-sensitivity mass spectrometry.
| Limit of Detection (LOD) | below 0.1 ng/mL |
|---|---|
| Calibration range | 0.2 to 20 ng/mL |
| Correlation coefficient (R²) | 0.999 or higher |
| Flow rate | 0.8 mL/min |
| Injection volume | 10 µL |
The method allows for a limit of detection below 0.1 ng/mL for both analytes.
The primary transition is 468.2 > 55.1, with a qualifier transition of 468.3 > 396.2.
The optimum wash is 2 x 2 mL of 2% formic acid, followed by 3 mL of 70% MeOH:30% 2% formic acid.