This document introduces the method of optimizing the method parameters of detecting steroids in wastewater using Agilent 6490 Triple Quadrupole LC/MS System with iFunnel Technology, resulting in a sensitivity as low as sub-1 ng/L (1 part per trillion).
This document describes a method for optimizing the detection and quantification of steroids in wastewater using the Agilent 6490 Triple Quadrupole LC/MS System. The study addresses the need for sensitive analysis to monitor environmental contaminants like pharmaceuticals that are not fully removed by sewage treatment plants.
The note provides specific experimental procedures, including sample preparation, cleanup via solid-phase extraction and normal phase chromatography, and detailed instrument conditions. Key optimization parameters discussed include Collision Cell Accelerator Voltage (CAV) and Multiplier Voltage (Delta EMV), which were adjusted to maximize ion response and achieve detection limits as low as sub-1 ng/L.
Detection limits as low as sub-1 ng/L (one part per trillion) were demonstrated.
The optimized CAV was found to be 2 volts, which significantly increased response compared to 5 volts.
The optimized value was found to be around 200-300 volts.