This application note describes an ultrafast SPE/MS/MS method, using the Agilent RapidFire High-Throughput Mass Spectrometry System, with a sample analysis time of <10 seconds per sample, for the analysis of levetiracetam in human serum. The Agilent RapidFire/MS System exhibited comparable accuracy, precision, linearity, and sensitivity to LC/MS/MS, but with a sample analysis cycle time 10-fold faster than LC/MS/MS.
This document evaluates the Agilent RapidFire High-Throughput Mass Spectrometry System for analyzing levetiracetam in human serum. The study demonstrates that the system achieves analysis times of less than 10 seconds per sample, offering a 10-fold speed increase over traditional LC/MS/MS methods while maintaining comparable accuracy, precision, and sensitivity.
The RapidFire/MS/MS system consists of an Agilent RapidFire 360, an Agilent 6460 Triple Quadrupole LC/MS System, and specific software. The document details the experimental conditions, sample preparation procedures, and results showing excellent linearity and low carryover, confirming the system's suitability for high-throughput analysis of similar assays.
| Analysis time per sample | < 10 seconds |
|---|---|
| Sample analysis cycle time | 9.5 seconds |
| Linear range | 1 pg/mL to 100 pg/mL |
| Correlation coefficient (R²) | > 0.995 |
| Signal-to-noise ratio | > 20:1 |
| Carryover | < 1% |
The system is capable of analysis times of less than 10 seconds per sample.
Yes, the system had comparable accuracy, precision, linearity, and sensitivity to LC/MS/MS.