This application note describes a rapid method for detecting benzoylecgonine (BZE), the major metabolite of cocaine, in urine using the Agilent RapidFire High-throughput Mass Spectrometry System. Results were achieved with much faster sample cycle times and similar analytical output compared to GC/MS or LC/MS assays.
This document presents a study evaluating the Agilent RapidFire High-Throughput Mass Spectrometry System for analyzing benzoylecgonine (BZE), the major metabolite of cocaine, in urine samples. The system is capable of analyzing samples with cycle times of less than 15 seconds, offering high throughput compared to traditional GC/MS or LC/MS assays.
The study details the experimental setup, including the use of an Agilent RapidFire 360 coupled with an Agilent 6460 Triple Quadrupole Mass Spectrometer. It outlines the specific chemical conditions, sample preparation methods involving 96-well plates, and data analysis procedures using MassHunter and RapidFire Integrator software. Results demonstrate accurate and precise measurement of BZE with a limit of detection less than 2 ng/mL and a throughput greater than 240 samples per hour.
| Sample cycle time | less than 15 seconds |
|---|---|
| Throughput | greater than 240 samples per hour |
| Limit of detection (LOD) | less than 2 ng/mL |
| Injection volume | 10 pL |
| Gas temperature | 350 °C |
| Sheath gas temperature | 400 °C |
| Nozzle voltage | 300 V |
| Capillary voltage | 3,500 V |
The system is capable of analyzing samples with cycle times of less than 15 seconds.
The limit of detection (LOD) for BZE was determined to be less than 2 ng/mL.
The methodology is capable of throughputs greater than 240 samples per hour.