The document provides information about the XL1-Blue MRF' Kan Library Pack Competent Cells, including the provided materials and storage conditions.
This document provides product details for the Agilent Technologies XL1-Blue MRF' Kan Library Pack Competent Cells (Catalog Number 200138). The pack contains 5 x 500 µl of competent cells optimized for cDNA library construction using the BacterioMatch two-hybrid system. It also includes a pUC18 control plasmid and B-Mercaptoethanol.
| Contents Volume | 5 x 500 µl |
|---|---|
| Storage Temperature | -80°C |
| Transformation Efficiency (Control) | 21.0 x 10^9 cfu/µg pUC18 DNA |
| Control Plasmid Concentration | 0.1 ng/µl in TE buffer |
Competent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
No, competent cells are sensitive to temperature variations and should not be stored in liquid nitrogen.
It is important that 14-ml BD Falcon polypropylene round-bottom tubes (BD Biosciences Catalog #352059) are used.