The SpotReport® Alien® Array Validation System* is a group of controls that collectively validates microarray data. They can be used for any genome microarray and play a pivotal role in normalization, monitoring hybridization specificity, and checking overall data quality from each microarray.
The SpotReport Alien Array Validation System is a group of controls designed to validate microarray data. It consists of a carefully selected set of 10 artificial sequences with no sequence homology to any genome, available as oligos, PCR products, or amine-modified oligos. These controls serve as negative controls that can be spotted onto any microarray to monitor data quality and global expression changes.
| Number of artificial sequences | 10 |
|---|---|
| Sequence homology | No sequence homology with any genome |
| Available formats | Oligos, PCR products, amine-modified oligos |
| mRNA spike quantity | 100 ng each |
| Slides printed per kit | 500-1000 slides |
It is a group of controls that collectively validates microarray data by monitoring data quality and global expression changes.
It allows users to accurately normalize data and establish experimental quality and signal linearity using external controls.
By spotting several Alien control genes and spiking a range of concentrations of corresponding Alien mRNA to monitor variability in fluorescence intensities.