The Brilliant II and Brilliant II FAST SYBR QPCR Master Mixes are single-tube reagents designed for performing quantitative PCR amplifications with an accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes. The master mix formulations and fast cycling protocol were developed together to create a system for completing QPCR reactions in less time without compromising target detection sensitivity, specificity, or reproducibility. The Brilliant II QPCR Master Mixes have been successfully used with fluorescent TaqMan probes and SYBR Green to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA. The Brilliant II FAST QPCR kit includes 2× master mix containing hot start Taq DNA polymerase, dNTPs, Mg2+, and a buffer specially formulated for fast cycling. The Brilliant II FAST SYBR QPCR Master Mix also includes the DNA binding dye SYBR Green for detection. The DNA polymerase features a rapid hot start capability that reduces nonspecific product formation while keeping the run time of the PCR protocol to a minimum, making it ideal for the Brilliant II QPCR kits. Both Brilliant II FAST master mixes have been developed, tested, and validated on our Mx3000P and Mx3005P QPCR systems. By utilizing our Mx QPCR systems in combination with our new Brilliant II FAST QPCR kits
The Stratagene Brilliant II and Brilliant II FAST SYBR QPCR Master Mixes are single-tube reagents designed for performing quantitative PCR amplifications. The formulations utilize a fast cycling protocol to complete 40 cycles of PCR in approximately 48 minutes without compromising target detection sensitivity, specificity, or reproducibility.
The Brilliant II FAST QPCR kit includes a 2× master mix containing hot start Taq DNA polymerase, dNTPs, Mg2+, and a buffer specially formulated for fast cycling. The Brilliant II FAST SYBR QPCR Master Mix also includes the DNA binding dye SYBR Green for detection. These reagents have been developed, tested, and validated on Stratagene Mx3000P and Mx3005P QPCR systems.
The accelerated cycling protocol completes 40 cycles of PCR in approximately 48 minutes.
They have been used to amplify and detect genomic DNA, plasmid DNA, and cDNA.
It features a rapid hot start capability that reduces nonspecific product formation while keeping the run time to a minimum.