The document introduces different types of competent cells products from Stratagene, including electroporation-competent cells, subcloning-grade competent cells, HTE and HEE phenotypes, etc. These products feature high efficiency, ease of use, and cost-effectiveness.
This document serves as a comprehensive guide to Stratagene's extensive line of competent cells, designed to match specific cloning strategies and applications. The catalog covers various cell strains optimized for difficult cloning, protein expression, and general cloning needs, highlighting their transformation efficiencies and unique genotypes.
| XL10-Gold transformation efficiency | 25x10^9 cfu/ug pUC18 |
|---|---|
| SURE 2 transformation efficiency | 21x10^9 cfu/ug pUC18 |
| XL1-Blue transformation efficiency | >1x10^9 cfu/ug pUC18 |
XL10-Gold ultracompetent cells are designed to transform large plasmids and ligated DNA with the highest transformation efficiency possible.
XL10-Gold ultracompetent cells have a stated efficiency of 25 x 10^9 transformants per microgram of supercoiled DNA.
The assay used 500 ng of the pRK2013 plasmid, which is a 25 kb molecule.