AffinityScript ™ Multiple Temperature Reverse Transcriptase is an engineered enzyme with high affinity for primer template complexes and can synthesize cDNA across a broad range of temperatures from 37°C to 55°C. The enzyme promotes cDNA yield due to its high affinity for primer:template complexes.
The Stratagene AffinityScript™ Multiple Temperature Reverse Transcriptase is engineered for high affinity to primer template complexes and thermostability ranging from 37°C to 55°C. This enzyme allows for the production of high yields of full-length cDNA, outperforming SuperScript® II and III RTs in terms of yield and ability to synthesize long cDNA up to 20 kb.
| Optimal Temperature Range | 37°C to 55°C |
|---|---|
| Maximum cDNA Length | 20 kb |
| Affinity Comparison | 10-fold higher |
| Reaction Time (QPCR Kit) | 15 minutes |
The enzyme is engineered for thermostability from 37°C to 55°C.
It produces higher yields of amplifiable cDNA and synthesizes long cDNA better than SuperScript® II and III RTs.
It can produce full-length cDNA up to 20 kb.