This document presents updated protocols for the Stratagene RecoverEase DNA isolation kit to isolate and purify high-molecular-weight genomic DNA. The new procedures cover samples including blood, bone marrow, tissue culture cells, liver, lung, kidney, spleen, testes, and brain. These methods eliminate the need for toxic phenol or chloroform and require minimal hands-on time.
| Compatible Tissues | blood, bone marrow, cultured cells, liver, lung, kidney, spleen, testes, brain |
|---|---|
| Maximum Processing Time | less than 24 hours |
| DNA Size Capability | 500-kb long |
No, each procedure eliminates the need for toxic phenol or chloroform.
The isolation of cell nuclei by centrifugation takes less than 20 minutes.
The protease digestion of the nuclei requires 45 minutes.