SideStep ™ II QRT-PCR products allow you to skip RNA purification and go directly to QRT-PCR
This document describes the SideStep II Lysis and Stabilization Products, which allow users to quantify gene expression directly from cells without prior RNA purification. The kits utilize a single-tube format that stabilizes RNA in cell lysates for at least 20 months when stored at -80°C.
| RNA stabilization duration | at least 20 months at -80°C |
|---|---|
| Time to QRT-PCR readiness | 10 minutes |
| Normalization method | single-copy human DNA sequence |
No, the SideStep II QRT-PCR products allow you to skip RNA purification and go directly to QRT-PCR.
Samples must be stored at -80°C to maintain stability.
Yes, it includes three Quantos QPCR Normalization Primer Sets that amplify unique single-copy human DNA sequences.