Key facts
- Brand
- STRATAGENE
- Model
- XL1-BLUE
- Documents
- 3
- Languages
- EN
- Last added
- 2012-06-06
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Stratagene XL1-Blue MRF' Supercompetent Cells Transformation Protocol
The document details the contents of the XL1-Blue MRF' Supercompetent Cells kit, including the specific genotype of the cells which are deficient in restriction systems (McrA-, McrCB-hsdSMR-mrr-, HsdR-) and endonuclease activity. It specifies that the cells must be stored at -80°C immediately upon receipt from dry ice shipping and should not be stored in liquid nitrogen to maintain efficiency.
The guide outlines a step-by-step transformation protocol involving the use of 14-ml BD Falcon polypropylene round-bottom tubes, addition of beta-mercaptoethanol, heat pulsing at 42°C for 45 seconds, and recovery in SOC medium. It also describes conditions for blue-white color screening using pUC18 control DNA.
STRATAGENE XL1-BLUE specifications
| Contents Volume | 5 x 500 µl |
|---|---|
| Storage Temperature | -80°C |
| Transformation Efficiency (Control) | 21.0 x 10^9 cfu/µg pUC18 DNA |
| Control Plasmid Concentration | 0.1 ng/µl in TE buffer |
Source: Agilent XL1-Blue MRF' Kan Library Pack Competent Cells Product Information Sheet
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Questions answered in these manuals
What is the recommended storage condition for these cells?
Competent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
From: Agilent XL1-Blue MRF' Kan Library Pack Competent Cells Product Information Sheet
Can these cells be stored in liquid nitrogen?
No, competent cells are sensitive to temperature variations and should not be stored in liquid nitrogen.
From: Agilent XL1-Blue MRF' Kan Library Pack Competent Cells Product Information Sheet
What is the recommended storage temperature for these cells?
Competent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
From: Stratagene XL1-Blue MRF' Supercompetent Cells Transformation Protocol
Can these cells be stored in liquid nitrogen?
No, do not store the cells in liquid nitrogen as they are sensitive to temperature variations.
From: Stratagene XL1-Blue MRF' Supercompetent Cells Transformation Protocol
What are the storage conditions for these cells?
Competent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
From: Agilent Technologies XL1-Blue MR Supercompetent Cells Product Certificate
What is the guaranteed efficiency under test conditions?
The expected efficiency is 2.1 x 10^9 cfu/ug pUC18 DNA.
From: Agilent Technologies XL1-Blue MR Supercompetent Cells Product Certificate