The document introduces the innovation of viral gene delivery systems and the importance of viral-based gene expression studies. With the use of viral gene delivery systems, gene function studies can be conducted in hosts that are difficult to transfect using traditional transfection methods.
This document introduces Stratagene's line of viral-based gene delivery systems designed to facilitate exceptionally high-efficiency gene delivery in difficult or impossible-to-transfect hosts. The brochure compares three specific systems: the AAV Helper-Free System for long-term stable expression, the AdEasy XL Adenoviral System for transient high-level protein production, and the ViraPort Retroviral cDNA Expression System for functional cloning assays.
The system has a large cloning capacity of 8 kb when using the pFB vector.
Generating adenoviral vectors in E. coli using this system takes only three days.
The AAV Helper-Free System achieves a gene delivery efficiency greater than 90%.