XL10-Gold ultra-competent cells are ultra-high density, high quality, easy-to-use bacterial strains for high efficiency transformation of bacteria.
This document is a certificate of analysis for the Agilent Technologies XL10-Gold Ultracompetent Cells (Catalog Number 200314). It specifies that the product contains XL10-Gold ultracompetent cells, pUC18 control plasmid, and B-Mercaptoethanol mix. The cells are shipped on dry ice and must be stored immediately at -80°C in a freezer; storage in liquid nitrogen is prohibited.
| Control Plasmid Concentration | 0.1 ng/pil in TE buffer |
|---|---|
| B-Mercaptoethanol Volume | 50 pl |
| Storage Temperature | -80°C freezer |
| Guaranteed Efficiency | 25x 10° cfg pUC18 DNA |
| Antibiotic Resistance | Tetracycline and Chloramphenicol |
No, the document explicitly states not to store the cells in liquid nitrogen.
The optimal duration is 30 seconds at 42°C, as efficiency decreases sharply outside this range.
It is important to use 14-ml BD Falcon polypropylene round-bottom tubes.