STRATAGENE AccuScript High Fidelity Reverse Transcriptase Product Leaflet

AccuScript™ High Fidelity Reverse Transcriptase products are the best reverse transcriptase for first-strand cDNA synthesis, producing the highest transcription accuracy possible. It is the ideal choice for any application that requires premium error-free cDNA.


STRATAGENE AccuScript High Fidelity Reverse Transcriptase Product Leaflet - cover page
Brand
STRATAGENE
Category
Other products
Document type
Product Leaflet
Language
English
Pages
4
File format
PDF
File size
524 KB
Published
04 July, 2012
Updated
01 October, 2023
MD5 checksum
ADD05D9F38597BE11287989B8D2BD7FE

About this manual

This document introduces the AccuScript High Fidelity Reverse Transcriptase, an RNase H-deficient Moloney murine leukemia virus (MMLV) reverse transcriptase engineered for the highest transcription accuracy. It is designed to produce premium, error-free cDNA suitable for applications requiring high fidelity, such as gene cloning, sequencing, and protein expression. The product is noted for delivering 3- to 6-fold fewer errors compared to SuperScript II and III reverse transcriptases.

What's inside

  • Highest Accuracy RT
  • RNase H deficiency
  • Full-length cDNA synthesis
  • RT-PCR performance
  • Quality Control
  • Kit Formats
  • Error Rates
  • Nuclease-free Enzyme

Specifications

cDNA Length Capability1 to 20 kb
Enzyme TypeRNase H deficient MMLV RT
Accuracy vs Transcriptor4.7-fold more accurate

Frequently asked questions

What is the main advantage of AccuScript RT?

It delivers the highest transcription accuracy possible while promoting full-length cDNA synthesis.

What is the maximum length of cDNA that AccuScript Reverse Transcriptase can synthesize?

The product can synthesize full-length cDNAs up to 20 kb in length.

How many times more accurate is AccuScript Reverse Transcriptase compared to SuperScript II and SuperScript III?

It provides over 3.7-fold more accurate reverse transcription than other RTs such as SuperScript II and SuperScript III.

What is the predicted percentage of clones containing errors when using AccuScript RT with PfuUitra DNA Polymerase?

Fewer than 2.5% of the clones contain errors in this combination.

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