SoloPack Gold competent cells are a high-efficiency plasmid transformation kit, which includes 15 single-tube transformations (each 25 µl) and pUC18 control plasmid (0.1 ng/µl in TE buffer, 10 µl), can be stored at -80°C for a long time.
This document is a product certificate for Agilent Technologies' SoloPack Gold Competent Cells. It specifies that the cells are shipped on dry ice and must be stored at -80°C in an ultracompetent freezer immediately upon receipt. The cells are packaged as single-tube aliquots to facilitate high-performance transformations without the need for thawing or refreezing.
| Contents per pack | 15 single-tube transformations (25 pil each) |
|---|---|
| Control plasmid included | pUC18 control plasmid (0.1 ng/pil in TE buffer) |
| Antibiotic Resistance | Tetracycline and Chloramphenicol resistant |
| Endonuclease Status | endA deficient |
Ultracompetent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
Optimal efficiency is observed when cells are heat-pulsed at 42°C for 60 seconds in the tubes provided.
For the pUC18 control, expect 25 colonies (21 x 10° cfu/ug pUC18 DNA).