SoloPack Gold Supercompetent Cells are a cell line of Escherichia coli bacteria that are used to transform DNA into expression vectors, such as the pUC18 control plasmid.
This document is a product certificate for Agilent Technologies' SoloPack Gold supercompetent cells (Catalog Number 230350). It specifies that the cells are shipped on dry ice and must be stored immediately at -80°C in a freezer, avoiding liquid nitrogen. The package contains 15 single-tube transformations of 50 µl each.
| Contents per package | 15 single-tube transformations (50 pl each) |
|---|---|
| Control Plasmid Included | pUC18 control plasmid (0.1 ng/pil in TE buffer), 10 pl |
| Shipping Condition | Shipped on dry ice |
| Storage Temperature | -80°C freezer |
| Antibiotic Resistance | Tetracycline and Chloramphenicol |
| Heat Shock Temperature | 42°C |
| Heat Shock Duration | 30 seconds |
NZY broth provides maximum transformation efficiency, whereas SOC results in slightly lower efficiencies.
For the pUC18 control, expect 167 colonies (1 x 10° cfi/jg pUC18 DNA).