The document describes the features and characteristics of ABLE® K Electroporation-Competent Cells, including the provided materials and quality control testing.
The document describes the ABLE® K electroporation-competent cells (Catalog #200162), detailing their genotype, resistance properties, and efficiency. It specifies that these cells are designed to enhance the probability of retrieving clones toxic to E. coli by reducing plasmid copy number.
| Quantity per tube | 100 ul |
|---|---|
| Resistance | Kanamycin and tetracycline |
Transfer the cells directly from the dry ice shipping container to the bottom of a -80°C freezer. Do not store cells in liquid nitrogen.
Set the electroporator to a voltage setting of 1700 V (17 kV/cm field strength).
Add 1 ul of plasmid DNA (16 pg/ul) to 40 ul of cells.