The document describes the features and characteristics of XL1-Blue MR supercompetent cells. It provides the necessary materials for experiments and specifies the storage and shipping conditions. The product guarantees efficient transformations and includes a testing method for transformation efficiency.
This document is a certificate of analysis for Agilent Technologies XL1-Blue MR supercompetent cells (Catalog Number 200229). It specifies that the product contains 5 x 200 pl tubes of cells along with pUC18 control plasmid and B-Mercaptoethanol. The cells are shipped on dry ice and must be stored immediately at -80°C in a freezer, avoiding liquid nitrogen to maintain efficiency.
Competent cells must be placed immediately at the bottom of a -80°C freezer directly from the dry ice shipping container.
The expected efficiency is 2.1 x 10^9 cfu/ug pUC18 DNA.